Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4641100..4641716 Replicon chromosome
Accession NZ_CP048416
Organism Citrobacter freundii strain CitB

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag GZ999_RS21835 Protein ID WP_003028682.1
Coordinates 4641100..4641474 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag GZ999_RS21840 Protein ID WP_043018956.1
Coordinates 4641474..4641716 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ999_RS21820 4638603..4639505 + 903 WP_003847898.1 formate dehydrogenase O subunit beta -
GZ999_RS21825 4639502..4640137 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
GZ999_RS21830 4640134..4641063 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
GZ999_RS21835 4641100..4641474 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
GZ999_RS21840 4641474..4641716 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
GZ999_RS21845 4641922..4642830 + 909 WP_003847901.1 alpha/beta hydrolase -
GZ999_RS21850 4642981..4643922 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
GZ999_RS21855 4643967..4644404 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
GZ999_RS21860 4644401..4645273 - 873 WP_003028669.1 virulence factor BrkB family protein -
GZ999_RS21865 4645267..4645866 - 600 WP_003028663.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T149412 WP_003028682.1 NZ_CP048416:c4641474-4641100 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T149412 NZ_CP064094:3466149-3466325 [Bacillus subtilis]
GTGCTTGAGAAAATGGGTATCGTAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT149412 WP_043018956.1 NZ_CP048416:c4641716-4641474 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT149412 NZ_CP064094:c3466366-3466266 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References