Detailed information of TA system TA149409

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3748997..3749703
• Replicon: chromosome
• Accession: NZ_CP048416
• Organism: Citrobacter freundii strain CitB

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: GZ999_RS17695
• Protein ID: WP_039556156.1
• Coordinates: 3748997..3749365 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: GZ999_RS17700
• Protein ID: WP_039556157.1
• Coordinates: 3749386..3749703 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ999_RS17680	3744136..3745587	+	1452	WP_003838742.1	Na+/H+ antiporter NhaC	-
GZ999_RS17685	3745600..3746781	+	1182	WP_003838744.1	pyridoxal phosphate-dependent aminotransferase	-
GZ999_RS17690	3746947..3748425	-	1479	WP_032941279.1	hypothetical protein	-
GZ999_RS17695	3748997..3749365	-	369	WP_039556156.1	TA system toxin CbtA family protein	Toxin
GZ999_RS17700	3749386..3749703	-	318	WP_039556157.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZ999_RS17705	3749722..3749939	-	218	Protein_3465	DUF987 family protein	-
GZ999_RS17710	3749948..3750430	-	483	WP_039556158.1	DNA repair protein RadC	-
GZ999_RS17715	3750439..3750882	-	444	WP_096164809.1	antirestriction protein	-
GZ999_RS17720	3750913..3751734	-	822	WP_058647033.1	DUF945 domain-containing protein	-
GZ999_RS17725	3751854..3752327	-	474	WP_058647034.1	hypothetical protein	-
GZ999_RS17730	3752399..3752851	-	453	WP_089586936.1	hypothetical protein	-
GZ999_RS17735	3752887..3753603	-	717	WP_058647035.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3739862..3763225	23363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13715.99 Da
• Isoelectric Point: 8.7174

>T149409 WP_039556156.1 NZ_CP048416:c3749365-3748997 [Citrobacter freundii]
MKTLPATISRATKPCLSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETIIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T149409 NZ_CP064094:c3007871-3007692 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11901.43 Da
• Isoelectric Point: 6.6246

>AT149409 WP_039556157.1 NZ_CP048416:c3749703-3749386 [Citrobacter freundii]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASITGKFSDAECRKLDETFPHFISQMESMLTTGEMNPRHAHCVILYH
NGFTCEADTLGSCGYVYISVYPTQR

Nucleotide

• Length: 318 bp

>AT149409 NZ_CP064094:3007546-3007768 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC