Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3539120..3539740 Replicon chromosome
Accession NZ_CP048416
Organism Citrobacter freundii strain CitB

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GZ999_RS16745 Protein ID WP_002892050.1
Coordinates 3539522..3539740 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag GZ999_RS16740 Protein ID WP_003021733.1
Coordinates 3539120..3539494 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ999_RS16730 3534266..3535459 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GZ999_RS16735 3535482..3538631 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
GZ999_RS16740 3539120..3539494 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
GZ999_RS16745 3539522..3539740 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GZ999_RS16750 3540047..3540598 + 552 WP_032936967.1 maltose O-acetyltransferase -
GZ999_RS16755 3540715..3541185 + 471 WP_003021724.1 YlaC family protein -
GZ999_RS16760 3541264..3541404 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
GZ999_RS16765 3541406..3541666 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
GZ999_RS16770 3541855..3543408 + 1554 WP_048241431.1 EAL domain-containing protein -
GZ999_RS16775 3543460..3543813 - 354 WP_003831033.1 DUF1428 family protein -
GZ999_RS16780 3543878..3544507 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149408 WP_002892050.1 NZ_CP048416:3539522-3539740 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T149408 NZ_CP064094:c951599-951249 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT149408 WP_003021733.1 NZ_CP048416:3539120-3539494 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT149408 NZ_CP064094:c951885-951604 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References