Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 873518..874172 Replicon chromosome
Accession NZ_CP048416
Organism Citrobacter freundii strain CitB

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag GZ999_RS04240 Protein ID WP_003026936.1
Coordinates 873765..874172 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag GZ999_RS04235 Protein ID WP_003026938.1
Coordinates 873518..873784 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ999_RS04210 868719..870152 - 1434 WP_048217186.1 6-phospho-beta-glucosidase BglA -
GZ999_RS04215 870273..871001 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
GZ999_RS04220 871054..871365 + 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
GZ999_RS04225 871528..872187 + 660 WP_003838267.1 hemolysin III family protein -
GZ999_RS04230 872281..873261 - 981 WP_003838269.1 tRNA-modifying protein YgfZ -
GZ999_RS04235 873518..873784 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
GZ999_RS04240 873765..874172 + 408 WP_003026936.1 protein YgfX Toxin
GZ999_RS04245 874217..874738 - 522 WP_003026933.1 flavodoxin FldB -
GZ999_RS04250 874852..875748 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
GZ999_RS04255 875772..876485 + 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GZ999_RS04260 876491..878224 + 1734 WP_032937435.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T149398 WP_003026936.1 NZ_CP048416:873765-874172 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T149398 NZ_CP064092:3830754-3831104 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT149398 WP_003026938.1 NZ_CP048416:873518-873784 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT149398 NZ_CP064092:3830468-3830749 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References