Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 253954..254585 Replicon chromosome
Accession NZ_CP048416
Organism Citrobacter freundii strain CitB

Toxin (Protein)


Gene name hicA Uniprot ID A0A0J1MPQ6
Locus tag GZ999_RS01165 Protein ID WP_003837806.1
Coordinates 253954..254229 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0J1MMS0
Locus tag GZ999_RS01170 Protein ID WP_003837808.1
Coordinates 254226..254585 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ999_RS01155 250005..252752 + 2748 WP_032937231.1 ribosome-associated ATPase/putative transporter RbbA -
GZ999_RS01160 252752..253876 + 1125 WP_003837801.1 ABC transporter permease -
GZ999_RS01165 253954..254229 + 276 WP_003837806.1 type II toxin-antitoxin system HicA family toxin Toxin
GZ999_RS01170 254226..254585 + 360 WP_003837808.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GZ999_RS01175 254696..255097 - 402 WP_032937232.1 nickel-responsive transcriptional regulator NikR -
GZ999_RS01180 255147..255725 - 579 WP_003023390.1 4'-phosphopantetheinyl transferase AcpT -
GZ999_RS01185 255788..256837 - 1050 WP_003023393.1 AI-2E family transporter -
GZ999_RS01190 256971..258188 + 1218 WP_048217067.1 MFS transporter -
GZ999_RS01195 258192..258749 - 558 WP_003837816.1 DcrB family lipoprotein -
GZ999_RS01200 258822..259487 - 666 WP_003837818.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10210.88 Da        Isoelectric Point: 10.7228

>T149395 WP_003837806.1 NZ_CP048416:253954-254229 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Download         Length: 276 bp

>T149395 NZ_CP064091:3041409-3041759 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13198.98 Da        Isoelectric Point: 4.5462

>AT149395 WP_003837808.1 NZ_CP048416:254226-254585 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Download         Length: 360 bp

>AT149395 NZ_CP064091:3041122-3041403 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCACAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MPQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MMS0

References