149386

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5868487..5870213 Replicon chromosome
Accession NZ_CP048408
Organism Pseudomonas fluorescens strain DR397

Toxin (Protein)


Gene name pmenT Uniprot ID A0A2N8H983
Locus tag GZH78_RS26370 Protein ID WP_074691609.1
Coordinates 5868487..5869131 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A0C1WLD3
Locus tag GZH78_RS26375 Protein ID WP_039771896.1
Coordinates 5869128..5870213 (+) Length 362 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZH78_RS26345 5864298..5864825 + 528 WP_007990564.1 inorganic diphosphatase -
GZH78_RS26350 5865047..5865787 + 741 WP_039771890.1 helix-turn-helix transcriptional regulator -
GZH78_RS26355 5866265..5866672 + 408 WP_039772000.1 hypothetical protein -
GZH78_RS26360 5866803..5867723 + 921 WP_039771891.1 restriction endonuclease -
GZH78_RS26365 5867909..5868490 + 582 WP_103385497.1 DUF4433 domain-containing protein -
GZH78_RS26370 5868487..5869131 + 645 WP_074691609.1 DUF4433 domain-containing protein Toxin
GZH78_RS26375 5869128..5870213 + 1086 WP_039771896.1 macro domain-containing protein Antitoxin
GZH78_RS26380 5870401..5870958 + 558 WP_039771897.1 hypothetical protein -
GZH78_RS26385 5870974..5871408 + 435 WP_039772002.1 hypothetical protein -
GZH78_RS26390 5871432..5872244 - 813 WP_039771899.1 DUF2135 domain-containing protein -
GZH78_RS26395 5872248..5873867 - 1620 WP_163976574.1 DUF2300 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24914.33 Da        Isoelectric Point: 6.5693

>T149386 WP_074691609.1 NZ_CP048408:5868487-5869131 [Pseudomonas fluorescens]
MNYTSLNPEKALIWRIVHRDNLPWILDNGLHCANSEVQAPHYVNIGNVDLIDKRRSRQVPIAPEGVLADYVPFYFTPFSV
MMQNIHSGWSVQQRSNDEIVILVSSLHRVEELGLPFVFTNAHAYPSWTDYYSDLANLDQIDWTILQRRDFKRDPDDPRKI
ERYQAEALIHHHLPITGLLGIMCHTDAMKERIEQDVAARGLTLSVHARPKWYFQ

Download         Length: 645 bp

>T149386 NZ_CP064056:887351-887557 [Staphylococcus lloydii]
ATGGTAACAAAAGTAATTTTAGTAGCAGGTCTTAATACTGCTGAGCAGGCGGAATCGTTAACGCAACATTTATTAAATAT
TATAGGTGTTCACCAAGCTAATGTTGATAGTCAAAATAGTTCTATTCAAGTCACTTTTACTACACCGGCTAATTTAAACA
ATATAGAAAAAGAAATTTATGATAAAGGTTATACAATAATCTTTTAA

Antitoxin


Download         Length: 362 a.a.        Molecular weight: 40728.84 Da        Isoelectric Point: 6.2649

>AT149386 WP_039771896.1 NZ_CP048408:5869128-5870213 [Pseudomonas fluorescens]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAQNYRLYAAACKAKEVETGKMHVTATNELEGPRWIVNFPTK
RHWRSPSQMAWITEGLHDLRRFLIENDVKSVAIPPLGAGNGGLSWPEVREQIVEVLGDLDVDVLVFEPTNQYLNVAKRNG
VETLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEKLPSTENPLNLKFIAHKFGPYANRLEHLLNNLDGSYLH
CDKRISDAEITDVIWFDQDRKAFLQTYLKTEAREYSQALERTAELIDGFESPFGMELLATVDWLLIKEGVVPTVSAVREA
LRNWDGGGGAAVRKNKLFDDQALEIAINRLISSSFSPKIAA

Download         Length: 1086 bp

>AT149386 NZ_CP064056:887044-887337 [Staphylococcus lloydii]
ATGACTGAAGTATCTCAAGCCCATCACTCTACACAGACTAAAAATAATTTAAAATCACGTTTAAATCGAATTGAAGGTCA
AGTCAAAGCAATCAATCGTATGATTGATGAAGACGTTTATTGTGATGATGTGTTAACACAAATTAGAGCAACACGTTCAG
CTTTGAATAGTGTAGCCACTAAATTATTAGATCACCATATGAAGAGTTGTATTATGGAAAAGATTGATAATGGTGAGAAC
GAAGCAGCCATGGAGGAATTGTTAGTTACATTTCAAAAATTAATGAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N8H983

Antitoxin

Source ID Structure
AlphaFold DB A0A0C1WLD3

References