149382

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 2090442..2091481 Replicon chromosome
Accession NZ_CP048408
Organism Pseudomonas fluorescens strain DR397

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A0C2A0B7
Locus tag GZH78_RS09425 Protein ID WP_039769753.1
Coordinates 2090442..2091017 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID J2X4E7
Locus tag GZH78_RS09430 Protein ID WP_007959254.1
Coordinates 2091014..2091481 (-) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZH78_RS09400 2085768..2087756 + 1989 WP_064594418.1 heme lyase CcmF/NrfE family subunit -
GZH78_RS09405 2087753..2088289 + 537 WP_039769747.1 DsbE family thiol:disulfide interchange protein -
GZH78_RS09410 2088286..2088756 + 471 WP_011333089.1 cytochrome c-type biogenesis protein CcmH -
GZH78_RS09415 2088753..2089955 + 1203 WP_007959249.1 c-type cytochrome biogenesis protein CcmI -
GZH78_RS09420 2089981..2090388 + 408 WP_039769751.1 hypothetical protein -
GZH78_RS09425 2090442..2091017 - 576 WP_039769753.1 PIN domain-containing protein Toxin
GZH78_RS09430 2091014..2091481 - 468 WP_007959254.1 helix-turn-helix domain-containing protein Antitoxin
GZH78_RS09435 2091760..2092164 + 405 WP_011333093.1 type II toxin-antitoxin system HicB family antitoxin -
GZH78_RS09440 2092211..2093398 - 1188 WP_163974325.1 MFS transporter -
GZH78_RS09445 2093640..2094260 + 621 WP_163974326.1 hypothetical protein -
GZH78_RS09450 2094316..2095314 - 999 WP_085682963.1 sulfate ABC transporter substrate-binding protein -
GZH78_RS09455 2095420..2096244 - 825 WP_039769763.1 ion transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

SinI-like, DNA-binding domain

(84-130)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21614.95 Da        Isoelectric Point: 6.2191

>T149382 WP_039769753.1 NZ_CP048408:c2091017-2090442 [Pseudomonas fluorescens]
VRHSSFTALYDACVLYPAPLRDFLMWLALSGRFRARWTQEIHCEWKRNLLKNRPDLTMEQLDRTSGLMDQAIPDACVHDY
EDLIAGLTLPDCDDRHVLAAAIRCGASVIVTFNQKDFPCKTLEPFGIEAQHPDEFVDNLLGLDPAAVVAAAQKQRRQLKM
PAMDVEPFLDLLQRQGLVKSVQALRGYHAIL

Download         Length: 576 bp

>T149382 NZ_CP064054:905732-905959 [Morganella morganii subsp. morganii]
ATGGCAAGGACGATTGAACGTTCATCTCAGTTTAAAAAAGATTATAAACGGGAAGTTAAGGGTATTTACCGTATGATGTT
AAATGACAGTCTACAGAACATCCTGAATATATTGGTAAATGATCTCCCTATACCTGAAAAATACGCTGATCATCCGCTTA
AAGGAAATTGGCGTACATTTCGTGATTGCCACCTCTATCCTGATTTGATTTTGATTTATAAAAAATAA

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17272.70 Da        Isoelectric Point: 6.4962

>AT149382 WP_007959254.1 NZ_CP048408:c2091481-2091014 [Pseudomonas fluorescens]
MSTVIHPSISLPVEREVKAAIEGQRALAAYLATHFETQHIQIFDEQNEAHRVELPTSALRLLIDILAELAAGNAVQVVPV
HAELTTQEAADLLNVSRPHLIKLLESGELSYHKTGKHRRVRFADLMDYKTRRTTASEQAMALLAEQAQALRTGYE

Download         Length: 468 bp

>AT149382 NZ_CP064054:905476-905748 [Morganella morganii subsp. morganii]
GTGGAATATGTCAGAGCAAAAATTGATCCGAAGCTGAAAGCAGAAGCGATTGAAACGCTTGAATCGATGGGCTTAACAGT
GAGTGACGCGATAAGAATGTTGATGATTCAGATTGTTGAACAGAAAGCGATGCCATTTTGTGTGAAAAGTACACAATATC
GTCATGCTGCACGTACCCGGGCCGCAATAGAAGAGTTGGAGTGCGGGAAAGGCAAAAAATTTGACAGTATTTCAGATTTA
ATGGCGGATCTGCAGGATGGCAAGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C2A0B7

Antitoxin

Source ID Structure
AlphaFold DB J2X4E7

References