149380

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 705776..706365 Replicon chromosome
Accession NZ_CP048408
Organism Pseudomonas fluorescens strain DR397

Toxin (Protein)


Gene name PumA Uniprot ID A0A0C1WIU8
Locus tag GZH78_RS03175 Protein ID WP_039766452.1
Coordinates 705776..706081 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q3KJ92
Locus tag GZH78_RS03180 Protein ID WP_011332089.1
Coordinates 706078..706365 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZH78_RS03155 701792..702859 + 1068 WP_007952372.1 uroporphyrinogen decarboxylase -
GZH78_RS03160 702930..704204 - 1275 WP_039766449.1 MFS transporter -
GZH78_RS03165 704323..705219 + 897 WP_039766450.1 LysR family transcriptional regulator -
GZH78_RS03170 705220..705657 - 438 WP_103386966.1 excinuclease -
GZH78_RS03175 705776..706081 + 306 WP_039766452.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GZH78_RS03180 706078..706365 + 288 WP_011332089.1 putative addiction module antidote protein Antitoxin
GZH78_RS03185 706414..706854 - 441 WP_163973885.1 excinuclease -
GZH78_RS03190 706875..708110 - 1236 WP_085611127.1 beta-ketoacyl-ACP synthase -
GZH78_RS03195 708110..708838 - 729 WP_039766460.1 3-oxoacyl-ACP reductase FabG -
GZH78_RS03200 708831..709310 - 480 WP_039766461.1 hotdog family protein -
GZH78_RS03205 709307..710503 - 1197 WP_163973886.1 beta-ketoacyl-[acyl-carrier-protein] synthase family protein -
GZH78_RS03210 710500..710982 - 483 WP_163973887.1 DUF3261 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-91)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11483.10 Da        Isoelectric Point: 9.6535

>T149380 WP_039766452.1 NZ_CP048408:705776-706081 [Pseudomonas fluorescens]
MTEILRSSTFSSWLVKLADSRARMRIQVRIDRMAEGNFGDVKAIGEGISEARIDYGPGYRVYFMQQGRQLVILLCGGDKS
SQSRDIKQARLIAESWQEQNP

Download         Length: 306 bp

>T149380 NZ_CP048408:705776-706081 [Pseudomonas fluorescens]
ATGACTGAGATACTTCGAAGCTCCACATTCTCGAGCTGGCTTGTGAAGCTGGCTGATAGTCGCGCCAGGATGCGTATTCA
GGTGCGAATTGATCGTATGGCTGAGGGAAACTTCGGTGATGTCAAAGCCATTGGCGAAGGGATCAGCGAAGCCCGAATTG
ACTATGGCCCGGGATATCGGGTCTATTTCATGCAGCAAGGTCGTCAGCTGGTCATCCTCCTTTGTGGTGGAGACAAGAGC
AGTCAATCTCGAGACATCAAGCAAGCCAGGCTCATCGCGGAATCCTGGCAGGAGCAAAACCCATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10446.86 Da        Isoelectric Point: 4.6412

>AT149380 WP_011332089.1 NZ_CP048408:706078-706365 [Pseudomonas fluorescens]
MTEQFTRWDSAEYLKTEEDMANYLDACMEEAGDDPAFIAKALGTIARARGMTQVARDAGLSRESLYRALSGEVNPEFGTI
LKVMKALGLKLHAST

Download         Length: 288 bp

>AT149380 NZ_CP048408:706078-706365 [Pseudomonas fluorescens]
ATGACCGAACAATTCACTCGTTGGGACTCAGCCGAGTACCTCAAGACAGAAGAGGACATGGCCAACTATCTGGATGCCTG
CATGGAAGAGGCGGGGGATGACCCGGCTTTTATTGCCAAGGCACTTGGCACAATTGCTCGGGCGCGTGGCATGACCCAGG
TCGCGCGGGATGCGGGGCTATCAAGGGAAAGTCTTTATCGCGCACTGTCAGGCGAGGTAAATCCCGAGTTTGGAACGATC
CTCAAAGTCATGAAAGCGCTGGGGCTGAAGTTGCATGCCAGCACATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C1WIU8

Antitoxin

Source ID Structure
AlphaFold DB A0A345RJ97

References