Detailed information of TA system TA149378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 138058..139685
• Replicon: chromosome
• Accession: NZ_CP048408
• Organism: Pseudomonas fluorescens strain DR397

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GZH78_RS00665
• Protein ID: WP_163973746.1
• Coordinates: 138333..139685 (-)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0C2A7P8
• Locus tag: GZH78_RS00660
• Protein ID: WP_039765075.1
• Coordinates: 138058..138336 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH78_RS00640	134602..136170	-	1569	WP_163973742.1	PLP-dependent aminotransferase family protein	-
GZH78_RS00645	136263..136886	+	624	WP_163973743.1	FMN-binding negative transcriptional regulator	-
GZH78_RS00650	136897..137343	+	447	WP_163973744.1	GNAT family N-acetyltransferase	-
GZH78_RS00655	137356..138027	+	672	WP_163973745.1	GNAT family N-acetyltransferase	-
GZH78_RS00660	138058..138336	-	279	WP_039765075.1	helix-turn-helix domain-containing protein	Antitoxin
GZH78_RS00665	138333..139685	-	1353	WP_163973746.1	HipA domain-containing protein	Toxin
GZH78_RS00670	139926..142883	+	2958	WP_163973747.1	autotransporter domain-containing protein	-
GZH78_RS00675	143084..144550	+	1467	WP_163973748.1	autotransporter domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 50045.51 Da
• Isoelectric Point: 4.8314

>T149378 WP_163973746.1 NZ_CP048408:c139685-138333 [Pseudomonas fluorescens]
MHNLTLQIFDAGKWHDAMVLSFDSPEKGFESRCSFGYESAYLVENIEAIGSPFSKAVSALFPLDWEGRRSITPAFVHDIA
PAGAAKRFLLARLGQEKPAGISADLYLLGRCTPAPIGNMRIKESAEAVDERKPIGFQRREVVSRDHRFLEYAYEMGAAIG
GATGAGGEAPKILLAEDHAGLLYPDAVLDDENVHQHWFVKFARNKGSQTDQDILRSEFHYYQALQALGIETVAAEGLALE
EANKPSLWMQRFDRQVTSLGVERFAVESIYSLAEVTTPGSAMNHMEVIRMLAELWRNAGQADQIPALVADYLRRDLINKI
LGNSDNHGRNTAIIRDEGSYRLAPIYDLAPMVMDDEGVTRTTKWPKELERAGDVDWRGVCRALADISDPDDPLAGLSDAP
ESFERLREDAQCLAELPDILSASGLPDRTMNHPGVHLKNLEQRLKEWDLK

Nucleotide

• Length: 1353 bp

>T149378 NZ_CP064050:c204790-204518 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10303.04 Da
• Isoelectric Point: 10.7364

>AT149378 WP_039765075.1 NZ_CP048408:c138336-138058 [Pseudomonas fluorescens]
MSMTVVERTVLIESIQEALAEGTVEIGEAVRRLRVEVTGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
KMGVVRVRRNNS

Nucleotide

• Length: 279 bp

>AT149378 NZ_CP064050:c205053-204790 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0C2A7P8