Detailed information of TA system TA149377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 5341630..5342500
• Replicon: chromosome
• Accession: NZ_CP048406
• Organism: Mesorhizobium sp. AA22

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A8T8D066
• Locus tag: A9K68_RS25530
• Protein ID: WP_065006219.1
• Coordinates: 5341630..5342136 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8T8D0Z6
• Locus tag: A9K68_RS25535
• Protein ID: WP_029355607.1
• Coordinates: 5342129..5342500 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K68_RS25515	5338101..5338985	-	885	WP_029355617.1	cytochrome c oxidase subunit II	-
A9K68_RS25520	5339361..5339930	+	570	WP_065006221.1	invasion associated locus B family protein	-
A9K68_RS25525	5340065..5341477	+	1413	WP_065006220.1	metalloprotease TldD	-
A9K68_RS25530	5341630..5342136	-	507	WP_065006219.1	RES domain-containing protein	Toxin
A9K68_RS25535	5342129..5342500	-	372	WP_029355607.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
A9K68_RS25540	5342586..5343827	-	1242	WP_065006218.1	class I SAM-dependent RNA methyltransferase	-
A9K68_RS25545	5343824..5344618	-	795	WP_065006217.1	TlyA family RNA methyltransferase	-
A9K68_RS25550	5344784..5345185	+	402	WP_065006216.1	GFA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (8-161)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18786.38 Da
• Isoelectric Point: 7.0505

>T149377 WP_065006219.1 NZ_CP048406:c5342136-5341630 [Mesorhizobium sp. AA22]
MPDSRLSLWRAFVPRWAHLPLSGEGAARFGGRWNPVGTPTIYAARELSTAWAEYNQGFVQHPALIAQLELSGARLADLTD
ADVLTKLGVTADIHRCEWRDIMDQGLVPETHTLRDRLIADGQHGVIYPSFMSPGGTCVALWRWNENNAPRLKVIDPDHRL
PKTPASWL

Nucleotide

• Length: 507 bp

>T149377 NZ_CP064050:17074-17331 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13222.33 Da
• Isoelectric Point: 10.2173

>AT149377 WP_029355607.1 NZ_CP048406:c5342500-5342129 [Mesorhizobium sp. AA22]
MNAAALEIRASRFGEERTPFLSARRVAELLGVNLTELAGLIGVARNTLAAKTGARKVDAALSPIVRILAMAGEMAGDEQR
AAIWFKHQPIPGWAGKTAYDLVREGKTDKVLAYLEAVRSGIYA

Nucleotide

• Length: 372 bp

>AT149377 NZ_CP064050:16812-17081 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA