Detailed information of TA system TA149373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3509715..3510364
• Replicon: chromosome
• Accession: NZ_CP048404
• Organism: Proteus mirabilis strain N18-00201

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: GYM49_RS16120
• Protein ID: WP_012368534.1
• Coordinates: 3509715..3510134 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: GYM49_RS16125
• Protein ID: WP_012368535.1
• Coordinates: 3510131..3510364 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYM49_RS16090	3505721..3506635	-	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
GYM49_RS16095	3506659..3507096	-	438	WP_004246810.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GYM49_RS16100	3507177..3507794	-	618	WP_020946398.1	glucose-1-phosphatase	-
GYM49_RS16105	3508136..3508444	-	309	WP_004246808.1	helix-turn-helix transcriptional regulator	-
GYM49_RS16110	3508773..3509189	-	417	WP_049196823.1	hypothetical protein	-
GYM49_RS16115	3509167..3509490	-	324	WP_012368533.1	hypothetical protein	-
GYM49_RS16120	3509715..3510134	-	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
GYM49_RS16125	3510131..3510364	-	234	WP_012368535.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GYM49_RS17590	3510632..3510784	+	153	WP_167552341.1	hypothetical protein	-
GYM49_RS16130	3511115..3512950	-	1836	WP_004246802.1	ribosome-dependent GTPase TypA	-
GYM49_RS16135	3513310..3514719	+	1410	WP_004246801.1	glutamate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T149373 WP_012368534.1 NZ_CP048404:c3510134-3509715 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T149373 NZ_CP064047:5908-6390 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT149373 WP_012368535.1 NZ_CP048404:c3510364-3510131 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT149373 NZ_CP064047:5654-5920 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0