Detailed information of TA system TA149372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3488287..3488986
• Replicon: chromosome
• Accession: NZ_CP048404
• Organism: Proteus mirabilis strain N18-00201

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GYM49_RS16005
• Protein ID: WP_163806902.1
• Coordinates: 3488287..3488673 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: GYM49_RS16010
• Protein ID: WP_004246828.1
• Coordinates: 3488666..3488986 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYM49_RS15990	3484306..3484887	-	582	WP_020946389.1	DNA-3-methyladenine glycosylase I	-
GYM49_RS15995	3485138..3486043	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
GYM49_RS16000	3486053..3488125	+	2073	WP_041701373.1	glycine--tRNA ligase subunit beta	-
GYM49_RS16005	3488287..3488673	+	387	WP_163806902.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYM49_RS16010	3488666..3488986	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
GYM49_RS16015	3489035..3489469	-	435	WP_036972158.1	hypothetical protein	-
GYM49_RS16020	3489462..3490346	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
GYM49_RS16025	3490564..3491850	-	1287	WP_049220287.1	DUF3748 domain-containing protein	-
GYM49_RS16030	3491987..3492604	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
GYM49_RS16035	3492906..3493529	+	624	WP_004246821.1	guanylate kinase	-
GYM49_RS16040	3493584..3493859	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14322.69 Da
• Isoelectric Point: 10.0642

>T149372 WP_163806902.1 NZ_CP048404:3488287-3488673 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNVNQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T149372 NZ_CP064046:c5248914-5248696 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT149372 WP_004246828.1 NZ_CP048404:3488666-3488986 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT149372 NZ_CP064046:c5249315-5248941 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: B4EZ97