Detailed information of TA system TA149369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1555916..1557560
• Replicon: chromosome
• Accession: NZ_CP048402
• Organism: Pasteurella multocida strain HS_Canada1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GV127_RS07110
• Protein ID: WP_163884139.1
• Coordinates: 1556253..1557560 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V4NAB0
• Locus tag: GV127_RS07105
• Protein ID: WP_005752888.1
• Coordinates: 1555916..1556251 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV127_RS07080	1551994..1552653	+	660	WP_163884136.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-
GV127_RS07085	1552717..1553286	+	570	WP_005755466.1	Der GTPase-activating protein YihI	-
GV127_RS07090	1553292..1553720	+	429	WP_005724851.1	DUF2489 domain-containing protein	-
GV127_RS07095	1553744..1555111	+	1368	WP_163884137.1	oxygen-independent coproporphyrinogen III oxidase	-
GV127_RS07100	1555152..1555820	+	669	WP_163884138.1	NAD(P)H-dependent oxidoreductase	-
GV127_RS07105	1555916..1556251	-	336	WP_005752888.1	helix-turn-helix domain-containing protein	Antitoxin
GV127_RS07110	1556253..1557560	-	1308	WP_163884139.1	HipA domain-containing protein	Toxin
GV127_RS07115	1557779..1558915	+	1137	WP_163884298.1	anhydro-N-acetylmuramic acid kinase	-
GV127_RS07120	1558981..1561563	-	2583	WP_059245702.1	DNA mismatch repair protein MutS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-375)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 50496.28 Da
• Isoelectric Point: 4.9118

>T149369 WP_163884139.1 NZ_CP048402:c1557560-1556253 [Pasteurella multocida]
MEKLTLQIYLEQAWCDLAFLYFDSETYQLCNFEYLTDYVVQHYGKTGEHAVSLNYPVNVFIDDTTSLWSWLSDIAPAGAS
RRYWLEALDIKQESEEKQQFLLLKHAAISPIGNLRIKDAVPEQVEVRLFDLDKAKDRQSDFLEYVNQSGAMVGGATGAGG
EAPKLLLKRRENQVWIDNQQQGQDQDVSYLVKYPRGNYSPIDCDILRAEYHYYQELAQLGFDTIDTTRMRLEEGKRYPSL
WLPRFDIVQREGKTHRLAFESVYSLLQKQGGVLDHETALRQLIERISTARLQFAQTDFVIEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQRIWLAPIYDFAPMRADPEGIIRTTTWQTKGEYPQEFAGEYHFERIAEVLADLVEPTLLMQELRRLAGSLVD
LKSRLVQRNLPQSILDFPAIGFDYLPEKLKKWGLI

Nucleotide

• Length: 1308 bp

>T149369 NZ_CP064046:c2046409-2046307 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12520.60 Da
• Isoelectric Point: 10.5259

>AT149369 WP_005752888.1 NZ_CP048402:c1556251-1555916 [Pasteurella multocida]
MQITRRNASTKGASVAEIDRKKVRNEIIQAMLMGELSQGEALRKLRIEMFGINQQHYIQLVKVSRQTLSNIENDKGNYSV
ETINKVFKPFGLELGLVPVSKALLKELFSTA

Nucleotide

• Length: 336 bp

>AT149369 NZ_CP064046:2046303-2046450 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Structures

Antitoxin

• AlphaFold DB: V4NAB0