Detailed information of TA system TA149367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 147..621
• Replicon: plasmid pKPS01_p4
• Accession: NZ_CP048393
• Organism: Klebsiella pneumoniae strain S01

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: GZH33_RS27390
• Protein ID: WP_064183949.1
• Coordinates: 147..413 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: GZH33_RS27395
• Protein ID: WP_064183950.1
• Coordinates: 397..621 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH33_RS27385 (GZH33_27895)	1..5101	-	5101	WP_074377680.1	hypothetical protein	-
GZH33_RS27390 (GZH33_27900)	147..413	-	267	WP_064183949.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZH33_RS27395 (GZH33_27905)	397..621	-	225	WP_064183950.1	TraY domain-containing protein	Antitoxin
GZH33_RS27400 (GZH33_27910)	780..1028	+	249	WP_074384346.1	hypothetical protein	-
GZH33_RS27405 (GZH33_27915)	1776..2177	-	402	WP_117080885.1	helix-turn-helix domain-containing protein	-
GZH33_RS27410 (GZH33_27920)	2177..2368	-	192	WP_004187666.1	Rop family plasmid primer RNA-binding protein	-
GZH33_RS27415 (GZH33_27925)	2786..2936	+	151	Protein_6	replication initiation protein	-
GZH33_RS27420 (GZH33_27930)	3183..4052	-	870	WP_110192038.1	WYL domain-containing protein	-
GZH33_RS27425 (GZH33_27935)	4680..4910	+	231	WP_074377679.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5101	5101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10415.06 Da
• Isoelectric Point: 7.9924

>T149367 WP_064183949.1 NZ_CP048393:c413-147 [Klebsiella pneumoniae]
MVWTINYSERALKSLRKMDKQTARRILDFMDLRIAVAEDPRQSGKPLKGELGEFWRYRVGDYRILCEILDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T149367 NZ_CP064045:5908-6390 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8307.39 Da
• Isoelectric Point: 5.7065

>AT149367 WP_064183950.1 NZ_CP048393:c621-397 [Klebsiella pneumoniae]
MLAIRLSAEIESRLDLLAKKTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEAVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT149367 NZ_CP064045:5654-5920 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA