Detailed information of TA system TA149365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 69467..70110
• Replicon: plasmid pKPS01_p1
• Accession: NZ_CP048390
• Organism: Klebsiella pneumoniae strain S01

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: GZH33_RS25865
• Protein ID: WP_001044768.1
• Coordinates: 69467..69883 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: GZH33_RS25870
• Protein ID: WP_001261287.1
• Coordinates: 69880..70110 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH33_RS25845 (GZH33_26305)	64847..65815	+	969	WP_161400566.1	IS5-like element IS903 family transposase	-
GZH33_RS25850 (GZH33_26310)	65966..66556	-	591	WP_000194575.1	hypothetical protein	-
GZH33_RS25855 (GZH33_26315)	66556..66813	-	258	WP_000343085.1	hypothetical protein	-
GZH33_RS25860 (GZH33_26320)	67167..69305	+	2139	WP_000350638.1	AAA family ATPase	-
GZH33_RS25865 (GZH33_26325)	69467..69883	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
GZH33_RS25870 (GZH33_26330)	69880..70110	-	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GZH33_RS25875 (GZH33_26335)	70406..70678	+	273	WP_061602529.1	hypothetical protein	-
GZH33_RS25880 (GZH33_26340)	70725..71429	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
GZH33_RS25885 (GZH33_26345)	71720..72724	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
GZH33_RS25890 (GZH33_26350)	72803..73237	-	435	WP_000429836.1	Hg(II)-responsive transcriptional regulator	-
GZH33_RS25895 (GZH33_26355)	73309..73659	+	351	WP_001294663.1	mercuric transport protein MerT	-
GZH33_RS25900 (GZH33_26360)	73673..73948	+	276	WP_000732292.1	mercury resistance system periplasmic binding protein MerP	-
GZH33_RS25905 (GZH33_26365)	73984..74406	+	423	WP_001340589.1	organomercurial transporter MerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / ant(3'')-Ia / aph(3')-Ia / mph(A)	-	1..205754	205754
	-	inside	IScluster/Tn	-	-	64847..72724	7877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T149365 WP_001044768.1 NZ_CP048390:c69883-69467 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T149365 NZ_CP064044:4533991-4534272 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT149365 WP_001261287.1 NZ_CP048390:c70110-69880 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT149365 NZ_CP064044:4533759-4534001 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGGCTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGAGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4