Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5238908..5239533 Replicon chromosome
Accession NZ_CP048389
Organism Klebsiella pneumoniae strain S01

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GZH33_RS25175 Protein ID WP_002882817.1
Coordinates 5238908..5239291 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GZH33_RS25180 Protein ID WP_004150355.1
Coordinates 5239291..5239533 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZH33_RS25160 (5236274) 5236274..5237176 + 903 WP_250354557.1 formate dehydrogenase subunit beta -
GZH33_RS25165 (5237173) 5237173..5237808 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GZH33_RS25170 (5237805) 5237805..5238734 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GZH33_RS25175 (5238908) 5238908..5239291 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GZH33_RS25180 (5239291) 5239291..5239533 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
GZH33_RS25185 (5239738) 5239738..5240655 + 918 WP_004181612.1 alpha/beta hydrolase -
GZH33_RS25190 (5240669) 5240669..5241610 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GZH33_RS25195 (5241655) 5241655..5242092 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
GZH33_RS25200 (5242089) 5242089..5242949 - 861 WP_004146232.1 virulence factor BrkB family protein -
GZH33_RS25205 (5242943) 5242943..5243542 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T149364 WP_002882817.1 NZ_CP048389:c5239291-5238908 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T149364 NZ_CP064044:c3173035-3172625 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT149364 WP_004150355.1 NZ_CP048389:c5239533-5239291 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT149364 NZ_CP064044:c3173282-3173016 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References