Detailed information of TA system TA149363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4925800..4927481
• Replicon: chromosome
• Accession: NZ_CP048389
• Organism: Klebsiella pneumoniae strain S01

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: GZH33_RS23735
• Protein ID: WP_004178299.1
• Coordinates: 4925800..4926717 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: GZH33_RS23740
• Protein ID: WP_004177809.1
• Coordinates: 4926723..4927481 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH33_RS23715 (4921106)	4921106..4922626	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
GZH33_RS23720 (4922623)	4922623..4923621	+	999	WP_002885172.1	ribose ABC transporter permease	-
GZH33_RS23725 (4923652)	4923652..4924593	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
GZH33_RS23730 (4924668)	4924668..4925675	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
GZH33_RS23735 (4925800)	4925800..4926717	-	918	WP_004178299.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GZH33_RS23740 (4926723)	4926723..4927481	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GZH33_RS23745 (4927567)	4927567..4928295	-	729	WP_004178298.1	response regulator transcription factor	-
GZH33_RS23750 (4928568)	4928568..4930715	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
GZH33_RS23755 (4930712)	4930712..4930855	-	144	WP_022631365.1	hypothetical protein	-
GZH33_RS23760 (4931293)	4931293..4931979	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34649.06 Da
• Isoelectric Point: 5.4707

>T149363 WP_004178299.1 NZ_CP048389:c4926717-4925800 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPSQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T149363 NZ_CP064044:c2046409-2046307 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT149363 WP_004177809.1 NZ_CP048389:c4927481-4926723 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT149363 NZ_CP064044:2046303-2046450 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Structures

Antitoxin

• AlphaFold DB: A6TH05