Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4670217..4671027 Replicon chromosome
Accession NZ_CP048389
Organism Klebsiella pneumoniae strain S01

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag GZH33_RS22545 Protein ID WP_004178461.1
Coordinates 4670217..4670750 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GZH33_RS22550 Protein ID WP_002887278.1
Coordinates 4670761..4671027 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZH33_RS22540 (4669048) 4669048..4670169 + 1122 WP_002887282.1 cupin domain-containing protein -
GZH33_RS22545 (4670217) 4670217..4670750 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
GZH33_RS22550 (4670761) 4670761..4671027 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
GZH33_RS22555 (4671130) 4671130..4672563 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
GZH33_RS22560 (4672553) 4672553..4673236 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
GZH33_RS22565 (4673408) 4673408..4674793 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
GZH33_RS22570 (4674811) 4674811..4675155 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T149361 WP_004178461.1 NZ_CP048389:c4670750-4670217 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T149361 NZ_CP064041:c1498546-1498268 [Vibrio parahaemolyticus]
ATGATTTTATGGGAAGAAGAATCGCTAAATGATCGTGAAAAGATCTTTGAGTTTCTTTATGACTTCAACCCTGACGCGGC
AGATAAAACTGACGAAATCATCGAAACTAAAGTAGAGAATTTACTTGAGCAACCACTGATGGGTGTTCAACGAGATGGTA
TTCGAGGGCGACTACTAATTATCCCCGAAATCTCGATGATTGTTTCCTACTGGGTTGACGGCTCAGTAATTCGTATAATG
CGAGTTCTACACCAGAAGCAGAAATTCCCTACCGACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT149361 WP_002887278.1 NZ_CP048389:c4671027-4670761 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT149361 NZ_CP064041:c1498827-1498543 [Vibrio parahaemolyticus]
ATGGACACTAGAATTCAATTTCGTGTTGATGACGAAGTTAAACGCTTAGCTCAACAAATGGCAGAGAGCCAAGGACGCAC
ACTTAGCGATGCTTGCCGTGAGCTTACCGAGCAAATGGCTGAACAACAACGAAAAGCATTATCTCACGACAATTGGTTGA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAAGCTTCATTTGTTGATCACGACTCAGCAAAAGCACGA
ATGGCTGAGCGAAAAGCTAAGATTCGAAACCGAGGTCAACAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References