Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4035906..4036525 Replicon chromosome
Accession NZ_CP048389
Organism Klebsiella pneumoniae strain S01

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GZH33_RS19545 Protein ID WP_002892050.1
Coordinates 4036307..4036525 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GZH33_RS19540 Protein ID WP_002892066.1
Coordinates 4035906..4036280 (+) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZH33_RS19530 (4031058) 4031058..4032251 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GZH33_RS19535 (4032274) 4032274..4035420 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
GZH33_RS19540 (4035906) 4035906..4036280 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GZH33_RS19545 (4036307) 4036307..4036525 + 219 WP_002892050.1 HHA domain-containing protein Toxin
GZH33_RS19550 (4036684) 4036684..4037250 + 567 WP_002892030.1 maltose O-acetyltransferase -
GZH33_RS19555 (4037222) 4037222..4037362 - 141 WP_004147370.1 hypothetical protein -
GZH33_RS19560 (4037383) 4037383..4037853 + 471 WP_002892026.1 YlaC family protein -
GZH33_RS19565 (4037828) 4037828..4039279 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
GZH33_RS19570 (4039380) 4039380..4040078 + 699 WP_002892021.1 GNAT family protein -
GZH33_RS19575 (4040075) 4040075..4040215 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GZH33_RS19580 (4040215) 4040215..4040478 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-69)

Antitoxin

(1-116)


Sequences


Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149360 WP_002892050.1 NZ_CP048389:4036307-4036525 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T149360 NZ_CP064037:2179153-2179431 [Vibrio parahaemolyticus]
ATGATTTTATGGGAAGAAGAATCGCTAAATGATCGTGAAAAGATCTTTGAGTTTCTTTATGACTTCAACCCTGACGCGGC
AGATAAAACTGACGAAATCATCGAAACTAAAGTAGAGAATTTACTTGAGCAACCACTGATGGGTGTTCAACGAGATGGTA
TTCGAGGGCGACTACTAATTATCCCCGAAATCTCGATGATTGTTTCCTACTGGGTTGACGGCTCAGTAATTCGTATAATG
CGAGTTCTACACCAGAAGCAGAAATTCCCTACCGACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT149360 WP_002892066.1 NZ_CP048389:4035906-4036280 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT149360 NZ_CP064037:2178872-2179156 [Vibrio parahaemolyticus]
ATGGACACTAGAATTCAATTTCGTGTTGATGACGAAGTTAAACGCTTAGCTCAACAAATGGCAGAGAGCCAAGGACGCAC
ACTTAGCGATGCTTGCCGTGAGCTTACCGAGCAAATGGCTGAACAACAACGAAAAGCATTATCTCACGACAATTGGTTGA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAAGCTTCATTTGTTGATCACGACTCAGCAAAAGCACGA
ATGGCTGAGCGAAAAGCTAAGATTCGAAACCGAGGTCAACAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References