Detailed information of TA system TA149358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2722890..2724478
• Replicon: chromosome
• Accession: NZ_CP048389
• Organism: Klebsiella pneumoniae strain S01

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GZH33_RS13250
• Protein ID: WP_004179815.1
• Coordinates: 2722890..2724212 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GZH33_RS13255
• Protein ID: WP_004176198.1
• Coordinates: 2724212..2724478 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH33_RS13235 (2717946)	2717946..2720447	+	2502	WP_250354547.1	fimbria/pilus outer membrane usher protein	-
GZH33_RS13240 (2720435)	2720435..2721439	+	1005	WP_004179822.1	fimbrial protein	-
GZH33_RS13245 (2721581)	2721581..2722873	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
GZH33_RS13250 (2722890)	2722890..2724212	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
GZH33_RS13255 (2724212)	2724212..2724478	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GZH33_RS13260 (2724744)	2724744..2725070	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
GZH33_RS13265 (2725067)	2725067..2725567	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
GZH33_RS13270 (2725545)	2725545..2726924	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
GZH33_RS13275 (2727270)	2727270..2728424	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
GZH33_RS13280 (2728405)	2728405..2729328	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2515580..2759313	243733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T149358 WP_004179815.1 NZ_CP048389:c2724212-2722890 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T149358 NZ_CP064035:c1943978-1943709 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTATTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTATGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT149358 WP_004176198.1 NZ_CP048389:c2724478-2724212 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT149358 NZ_CP064035:c1944225-1943971 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG