Detailed information of TA system TA149339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2899991..2900581
• Replicon: chromosome
• Accession: NZ_CP048388
• Organism: Citrobacter portucalensis strain PNUCL1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: GZH81_RS13720
• Protein ID: WP_212023891.1
• Coordinates: 2899991..2900323 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: GZH81_RS13725
• Protein ID: WP_212023893.1
• Coordinates: 2900324..2900581 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH81_RS13700 (2895893)	2895893..2897938	+	2046	WP_060682359.1	phenylacetic acid degradation bifunctional protein PaaZ	-
GZH81_RS13710 (2898257)	2898257..2899744	+	1488	WP_003846749.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
GZH81_RS13720 (2899991)	2899991..2900323	-	333	WP_212023891.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GZH81_RS13725 (2900324)	2900324..2900581	-	258	WP_212023893.1	antitoxin	Antitoxin
GZH81_RS13730 (2900929)	2900929..2901045	-	117	Protein_2701	helix-turn-helix domain-containing protein	-
GZH81_RS13740 (2901441)	2901441..2905505	-	4065	WP_212023895.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11696.57 Da
• Isoelectric Point: 8.5572

>T149339 WP_212023891.1 NZ_CP048388:c2900323-2899991 [Citrobacter portucalensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSPALFNKVTRLPIVVPVTSGGSFARAAGFTVSLDGSGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T149339 NZ_CP064004:c3436677-3436525 [Xanthomonas translucens pv. translucens]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTTTGTGCAGGCATGCTGACCGGCTGCAACACCATGGCCGGCGC
CGGTAAGGACATGCAGAAGGCCGGCGATAAGGTGGAAACCAAGGCCGACGACTGTAAAGACGGCAAGTGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9412.87 Da
• Isoelectric Point: 4.3690

>AT149339 WP_212023893.1 NZ_CP048388:c2900581-2900324 [Citrobacter portucalensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIENGCLIIEPQKRARYSLEELLAQCDPHAEISEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT149339 NZ_CP064004:c3436426-3436292 [Xanthomonas translucens pv. translucens]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA