Detailed information of TA system TA149338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2470441..2471177
• Replicon: chromosome
• Accession: NZ_CP048388
• Organism: Citrobacter portucalensis strain PNUCL1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GZH81_RS11570
• Protein ID: WP_212023648.1
• Coordinates: 2470695..2471177 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: GZH81_RS11565
• Protein ID: WP_008784769.1
• Coordinates: 2470441..2470707 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH81_RS11545 (2465811)	2465811..2466689	+	879	Protein_2268	outer membrane protein transport protein	-
GZH81_RS11550 (2466929)	2466929..2467558	+	630	WP_008784772.1	winged helix-turn-helix transcriptional regulator	-
GZH81_RS11555 (2467675)	2467675..2468061	-	387	WP_008784771.1	hypothetical protein	-
GZH81_RS11560 (2468305)	2468305..2470287	+	1983	WP_071692396.1	alkyl sulfatase dimerization domain-containing protein	-
GZH81_RS11565 (2470441)	2470441..2470707	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
GZH81_RS11570 (2470695)	2470695..2471177	+	483	WP_212023648.1	GNAT family N-acetyltransferase	Toxin
GZH81_RS11575 (2471475)	2471475..2471690	-	216	Protein_2274	fatty acid transporter	-
GZH81_RS11580 (2471765)	2471765..2473378	-	1614	WP_032943434.1	mechanosensitive ion channel	-
GZH81_RS11585 (2473759)	2473759..2474136	+	378	WP_020996403.1	hypothetical protein	-
GZH81_RS11590 (2474189)	2474189..2475025	+	837	WP_125363450.1	DMT family transporter	-
GZH81_RS11595 (2475035)	2475035..2475556	-	522	WP_048226507.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17541.39 Da
• Isoelectric Point: 9.8719

>T149338 WP_212023648.1 NZ_CP048388:2470695-2471177 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAKFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHVLTDEAKRFYLHHGFKASQLQERTLFLGLS

Nucleotide

• Length: 483 bp

>T149338 NZ_CP064004:c3436426-3436292 [Xanthomonas translucens pv. translucens]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT149338 WP_008784769.1 NZ_CP048388:2470441-2470707 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT149338 NZ_CP064004:c3436677-3436525 [Xanthomonas translucens pv. translucens]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTTTGTGCAGGCATGCTGACCGGCTGCAACACCATGGCCGGCGC
CGGTAAGGACATGCAGAAGGCCGGCGATAAGGTGGAAACCAAGGCCGACGACTGTAAAGACGGCAAGTGCTGA