Detailed information of TA system TA149336

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2115995..2116701
• Replicon: chromosome
• Accession: NZ_CP048388
• Organism: Citrobacter portucalensis strain PNUCL1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: GZH81_RS09920
• Protein ID: WP_212025539.1
• Coordinates: 2116333..2116701 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: GZH81_RS09915
• Protein ID: WP_212025537.1
• Coordinates: 2115995..2116312 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH81_RS09875 (2111271)	2111271..2112092	+	822	WP_212025522.1	DUF932 domain-containing protein	-
GZH81_RS09880 (2112309)	2112309..2113010	+	702	WP_050134901.1	WYL domain-containing protein	-
GZH81_RS09885 (2113049)	2113049..2113888	+	840	WP_212025525.1	hypothetical protein	-
GZH81_RS09890 (2113910)	2113910..2114383	+	474	WP_212025527.1	hypothetical protein	-
GZH81_RS09895 (2114461)	2114461..2114700	+	240	WP_212025529.1	DUF905 domain-containing protein	-
GZH81_RS09900 (2114797)	2114797..2115255	+	459	WP_212025531.1	antirestriction protein	-
GZH81_RS09905 (2115271)	2115271..2115747	+	477	WP_212025533.1	RadC family protein	-
GZH81_RS09910 (2115756)	2115756..2115977	+	222	WP_212025535.1	DUF987 domain-containing protein	-
GZH81_RS09915 (2115995)	2115995..2116312	+	318	WP_212025537.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZH81_RS09920 (2116333)	2116333..2116701	+	369	WP_212025539.1	TA system toxin CbtA family protein	Toxin
GZH81_RS09925 (2117062)	2117062..2117308	+	247	Protein_1945	Arm DNA-binding domain-containing protein	-
GZH81_RS09930 (2117863)	2117863..2120172	+	2310	WP_212025541.1	TerB N-terminal domain-containing protein	-
GZH81_RS09935 (2120176)	2120176..2121492	+	1317	WP_212025543.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2107931..2151462	43531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13815.09 Da
• Isoelectric Point: 8.8788

>T149336 WP_212025539.1 NZ_CP048388:2116333-2116701 [Citrobacter portucalensis]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLKDTPFCDQTVIQEHIDAGITLANAVNFLVEKNELIRIDRNGF
TLQERTPYLTVTDILHARRACGLMNSCSYREVSNIVLSRSRQ

Nucleotide

• Length: 369 bp

>T149336 NZ_CP064004:2579985-2580236 [Xanthomonas translucens pv. translucens]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGTGTCGTCAACAATCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGCCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTAGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11911.56 Da
• Isoelectric Point: 7.3231

>AT149336 WP_212025537.1 NZ_CP048388:2115995-2116312 [Citrobacter portucalensis]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASPTGKFSDAECLKLDVAFPHFIRQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTFGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT149336 NZ_CP064004:2580233-2580490 [Xanthomonas translucens pv. translucens]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACGGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTCTACAAGGTCGAGGCGGGCGTCCTACTGATCGCGCAGGTG
CGGTATCACTACGCGTAG