Detailed information of TA system TA149324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 234528..235429
• Replicon: plasmid p6_A
• Accession: NZ_CP048383
• Organism: Citrobacter freundii strain 62

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: GZS04_RS23865
• Protein ID: WP_008786589.1
• Coordinates: 234528..234986 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GZS04_RS23870
• Protein ID: WP_008786588.1
• Coordinates: 234983..235429 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS04_RS23820	230254..230496	+	243	WP_007372291.1	hypothetical protein	-
GZS04_RS23825	230507..230905	+	399	WP_007372292.1	hypothetical protein	-
GZS04_RS23830	230989..231315	+	327	WP_007372293.1	hypothetical protein	-
GZS04_RS23835	231678..232016	-	339	WP_007372294.1	hypothetical protein	-
GZS04_RS23840	232254..232697	+	444	WP_007372295.1	hypothetical protein	-
GZS04_RS23845	232816..233157	+	342	WP_007372296.1	hypothetical protein	-
GZS04_RS23850	233316..233870	+	555	WP_007372297.1	hypothetical protein	-
GZS04_RS23855	233920..234186	+	267	WP_007372298.1	hypothetical protein	-
GZS04_RS23860	234254..234484	-	231	WP_007372299.1	hypothetical protein	-
GZS04_RS23865	234528..234986	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
GZS04_RS23870	234983..235429	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
GZS04_RS23875	235578..235895	+	318	WP_016241555.1	hypothetical protein	-
GZS04_RS23880	235937..236779	+	843	WP_007372303.1	hypothetical protein	-
GZS04_RS23885	236751..237029	+	279	WP_007372304.1	hypothetical protein	-
GZS04_RS23890	237089..237532	+	444	WP_007372305.1	hypothetical protein	-
GZS04_RS25820	237819..237983	+	165	WP_007372306.1	hypothetical protein	-
GZS04_RS23895	238551..239393	+	843	WP_007372307.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..254268	254268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T149324 WP_008786589.1 NZ_CP048383:c234986-234528 [Citrobacter freundii]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T149324 NZ_CP064001:c1587832-1587581 [Xanthomonas translucens pv. translucens]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGTGTCGTCAACAATCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGCCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTAGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT149324 WP_008786588.1 NZ_CP048383:c235429-234983 [Citrobacter freundii]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT149324 NZ_CP064001:c1587584-1587327 [Xanthomonas translucens pv. translucens]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACGGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTCTACAAGGTCGAGGCGGGCGTCCTACTGATCGCGCAGGTG
CGGTATCACTACGCGTAG

Structures

Toxin

• AlphaFold DB: S1EZ83