Detailed information of TA system TA149323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 227229..227779
• Replicon: plasmid p6_A
• Accession: NZ_CP048383
• Organism: Citrobacter freundii strain 62

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: GZS04_RS23795
• Protein ID: WP_007372286.1
• Coordinates: 227471..227779 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: GZS04_RS23790
• Protein ID: WP_007372285.1
• Coordinates: 227229..227468 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS04_RS23750	222236..222631	+	396	WP_024196081.1	hypothetical protein	-
GZS04_RS23755	222813..223004	+	192	WP_016241560.1	hypothetical protein	-
GZS04_RS23760	223135..223830	+	696	WP_007372278.1	hypothetical protein	-
GZS04_RS23765	223853..224050	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
GZS04_RS23770	224103..224561	+	459	WP_008786599.1	hypothetical protein	-
GZS04_RS25810	224709..224849	+	141	WP_007372281.1	hypothetical protein	-
GZS04_RS23775	224865..225497	+	633	WP_007372282.1	hypothetical protein	-
GZS04_RS23780	225958..226629	+	672	WP_007372283.1	hypothetical protein	-
GZS04_RS25815	226626..227090	+	465	WP_008786596.1	hypothetical protein	-
GZS04_RS23790	227229..227468	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GZS04_RS23795	227471..227779	+	309	WP_007372286.1	CcdB family protein	Toxin
GZS04_RS23800	227800..227958	+	159	WP_016241558.1	hypothetical protein	-
GZS04_RS23805	228113..228667	+	555	WP_007372288.1	hypothetical protein	-
GZS04_RS23810	228737..229354	+	618	WP_007372289.1	hypothetical protein	-
GZS04_RS23815	229437..229907	+	471	WP_007372290.1	hypothetical protein	-
GZS04_RS23820	230254..230496	+	243	WP_007372291.1	hypothetical protein	-
GZS04_RS23825	230507..230905	+	399	WP_007372292.1	hypothetical protein	-
GZS04_RS23830	230989..231315	+	327	WP_007372293.1	hypothetical protein	-
GZS04_RS23835	231678..232016	-	339	WP_007372294.1	hypothetical protein	-
GZS04_RS23840	232254..232697	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..254268	254268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T149323 WP_007372286.1 NZ_CP048383:227471-227779 [Citrobacter freundii]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T149323 NZ_CP064001:c1587584-1587327 [Xanthomonas translucens pv. translucens]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACGGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTCTACAAGGTCGAGGCGGGCGTCCTACTGATCGCGCAGGTG
CGGTATCACTACGCGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT149323 WP_007372285.1 NZ_CP048383:227229-227468 [Citrobacter freundii]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT149323 NZ_CP064001:c1587832-1587581 [Xanthomonas translucens pv. translucens]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGTGTCGTCAACAATCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGCCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTAGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3