Detailed information of TA system TA149322

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 187411..188090
• Replicon: plasmid p6_A
• Accession: NZ_CP048383
• Organism: Citrobacter freundii strain 62

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: GZS04_RS23530
• Protein ID: WP_006812012.1
• Coordinates: 187749..188090 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: GZS04_RS23525
• Protein ID: WP_006812013.1
• Coordinates: 187411..187728 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS04_RS23480	182852..183673	+	822	WP_001548934.1	DUF945 domain-containing protein	-
GZS04_RS23485	183890..184591	+	702	WP_001548933.1	WYL domain-containing protein	-
GZS04_RS23490	184628..184864	+	237	WP_001115854.1	hypothetical protein	-
GZS04_RS23495	184864..185304	+	441	WP_103468661.1	hypothetical protein	-
GZS04_RS23500	185330..185797	+	468	WP_032200803.1	hypothetical protein	-
GZS04_RS23505	185874..186113	+	240	WP_000194654.1	DUF905 domain-containing protein	-
GZS04_RS23510	186211..186669	+	459	WP_000211838.1	antirestriction protein	-
GZS04_RS23515	186685..187161	+	477	WP_000811693.1	RadC family protein	-
GZS04_RS23520	187170..187391	+	222	WP_001548171.1	DUF987 domain-containing protein	-
GZS04_RS23525	187411..187728	+	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS04_RS23530	187749..188090	+	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
GZS04_RS23535	188292..188696	+	405	WP_007372197.1	FCD domain-containing protein	-
GZS04_RS23540	188723..189109	-	387	WP_009652914.1	plasmid stability protein	-
GZS04_RS23545	189123..190085	-	963	WP_007372199.1	plasmid stabilization protein	-
GZS04_RS23550	190372..191268	-	897	Protein_193	IS3 family transposase	-
GZS04_RS23555	191333..192484	+	1152	WP_001254932.1	IS30-like element IS30 family transposase	-
GZS04_RS23560	192494..192742	-	249	Protein_195	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..254268	254268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T149322 WP_006812012.1 NZ_CP048383:187749-188090 [Citrobacter freundii]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T149322 NZ_CP064001:732064-732198 [Xanthomonas translucens pv. translucens]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT149322 WP_006812013.1 NZ_CP048383:187411-187728 [Citrobacter freundii]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT149322 NZ_CP064001:731813-731965 [Xanthomonas translucens pv. translucens]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTTTGTGCAGGCATGCTGACCGGCTGCAACACCATGGCCGGCGC
CGGTAAGGACATGCAGAAGGCCGGCGATAAGGTGGAAACCAAGGCCGACGACTGTAAAGACGGCAAGTGCTGA

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2