Detailed information of TA system TA149313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4041475..4042114
• Replicon: chromosome
• Accession: NZ_CP048382
• Organism: Citrobacter freundii strain 62

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: GZS04_RS19420
• Protein ID: WP_003020221.1
• Coordinates: 4041938..4042114 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GZS04_RS19415
• Protein ID: WP_123924900.1
• Coordinates: 4041475..4041891 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS04_RS19395	4036521..4037465	-	945	WP_003843727.1	ABC transporter permease	-
GZS04_RS19400	4037466..4038482	-	1017	WP_058842662.1	ABC transporter ATP-binding protein	-
GZS04_RS19405	4038499..4039644	-	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
GZS04_RS19410	4039973..4041382	-	1410	WP_003843728.1	PLP-dependent aminotransferase family protein	-
GZS04_RS19415	4041475..4041891	-	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GZS04_RS19420	4041938..4042114	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
GZS04_RS19425	4042378..4043781	+	1404	WP_139935432.1	cytochrome ubiquinol oxidase subunit I	-
GZS04_RS19430	4043781..4044791	+	1011	WP_162648747.1	cytochrome d ubiquinol oxidase subunit II	-
GZS04_RS19435	4044791..4044916	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
GZS04_RS19440	4045106..4045336	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T149313 WP_003020221.1 NZ_CP048382:c4042114-4041938 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T149313 NZ_CP063997:c576137-575886 [Xanthomonas translucens pv. undulosa]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAAGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT149313 WP_123924900.1 NZ_CP048382:c4041891-4041475 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT149313 NZ_CP063997:c575889-575632 [Xanthomonas translucens pv. undulosa]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACTACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACCGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACATCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Structures

Toxin

• AlphaFold DB: A0A8B5QF36