Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3500072..3500662 Replicon chromosome
Accession NZ_CP048382
Organism Citrobacter freundii strain 62

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag GZS04_RS16705 Protein ID WP_003836692.1
Coordinates 3500330..3500662 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag GZS04_RS16700 Protein ID WP_003836694.1
Coordinates 3500072..3500329 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS04_RS16695 3495559..3499458 - 3900 WP_046670495.1 hypothetical protein -
GZS04_RS16700 3500072..3500329 + 258 WP_003836694.1 hypothetical protein Antitoxin
GZS04_RS16705 3500330..3500662 + 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GZS04_RS16715 3501137..3502054 + 918 WP_003030855.1 nitrogen assimilation transcriptional regulator NAC -
GZS04_RS16720 3502156..3503106 + 951 WP_003846751.1 HTH-type transcriptional regulator Cbl -
GZS04_RS16730 3503422..3504909 - 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T149312 WP_003836692.1 NZ_CP048382:3500330-3500662 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T149312 NZ_CP063997:c575889-575632 [Xanthomonas translucens pv. undulosa]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACTACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACCGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACATCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT149312 WP_003836694.1 NZ_CP048382:3500072-3500329 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT149312 NZ_CP063997:c576137-575886 [Xanthomonas translucens pv. undulosa]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAAGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References