Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2786515..2787135 Replicon chromosome
Accession NZ_CP048382
Organism Citrobacter freundii strain 62

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GZS04_RS13270 Protein ID WP_002892050.1
Coordinates 2786515..2786733 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag GZS04_RS13275 Protein ID WP_003021733.1
Coordinates 2786761..2787135 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS04_RS13235 2781748..2782377 + 630 WP_016149709.1 membrane protein -
GZS04_RS13240 2782442..2782795 + 354 WP_003831033.1 DUF1428 family protein -
GZS04_RS13245 2782847..2784400 - 1554 WP_162648392.1 EAL domain-containing protein -
GZS04_RS13250 2784589..2784849 + 261 WP_003835926.1 type B 50S ribosomal protein L31 -
GZS04_RS13255 2784851..2784991 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
GZS04_RS13260 2785070..2785540 - 471 WP_003021724.1 YlaC family protein -
GZS04_RS13265 2785657..2786208 - 552 WP_003835924.1 maltose O-acetyltransferase -
GZS04_RS13270 2786515..2786733 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GZS04_RS13275 2786761..2787135 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
GZS04_RS13280 2787624..2790773 - 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
GZS04_RS13285 2790796..2791989 - 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149311 WP_002892050.1 NZ_CP048382:c2786733-2786515 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T149311 NZ_CP063996:c3903097-3902945 [Xanthomonas translucens pv. undulosa]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTTTGTGCAGGCATGCTGACCGGCTGCAACACCATGGCCGGCGC
CGGTAAGGACATGCAGAAGGCCGGCGATAAGGTGGAAACCAAGGCCGACGACTGTAAAGACGGCAAGTGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT149311 WP_003021733.1 NZ_CP048382:c2787135-2786761 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT149311 NZ_CP063996:c3902846-3902712 [Xanthomonas translucens pv. undulosa]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References