Detailed information of TA system TA149308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1865519..1866185
• Replicon: chromosome
• Accession: NZ_CP048382
• Organism: Citrobacter freundii strain 62

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: GZS04_RS08930
• Protein ID: WP_046671413.1
• Coordinates: 1865519..1865878 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0D7LIR0
• Locus tag: GZS04_RS08935
• Protein ID: WP_003844682.1
• Coordinates: 1865868..1866185 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS04_RS08920	1862919..1864550	+	1632	WP_003031618.1	Na/Pi cotransporter family protein	-
GZS04_RS08925	1864616..1865305	-	690	WP_046671414.1	dipeptidase PepE	-
GZS04_RS08930	1865519..1865878	+	360	WP_046671413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZS04_RS08935	1865868..1866185	+	318	WP_003844682.1	helix-turn-helix domain-containing protein	Antitoxin
GZS04_RS08940	1866195..1866473	-	279	WP_046671412.1	putative addiction module antidote protein	-
GZS04_RS25715	1866877..1867038	-	162	WP_003841416.1	phage protein	-
GZS04_RS08945	1867180..1867536	+	357	WP_046671411.1	hypothetical protein	-
GZS04_RS08950	1867582..1868394	-	813	WP_044702264.1	shikimate 5-dehydrogenase	-
GZS04_RS08955	1868396..1869619	-	1224	WP_003031639.1	L-sorbose 1-phosphate reductase	-
GZS04_RS08960	1869687..1870511	-	825	WP_003031641.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13451.52 Da
• Isoelectric Point: 10.2555

>T149308 WP_046671413.1 NZ_CP048382:1865519-1865878 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAIYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T149308 NZ_CP063996:3179107-3179358 [Xanthomonas translucens pv. undulosa]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAAGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11466.35 Da
• Isoelectric Point: 9.0005

>AT149308 WP_003844682.1 NZ_CP048382:1865868-1866185 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT149308 NZ_CP063996:3179355-3179612 [Xanthomonas translucens pv. undulosa]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACTACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACCGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACATCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0D7LIR0