Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1714188..1714804 Replicon chromosome
Accession NZ_CP048382
Organism Citrobacter freundii strain 62

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag GZS04_RS08255 Protein ID WP_003028682.1
Coordinates 1714430..1714804 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag GZS04_RS08250 Protein ID WP_043018956.1
Coordinates 1714188..1714430 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS04_RS08225 1710038..1710637 + 600 WP_046671351.1 glucose-1-phosphatase -
GZS04_RS08230 1710631..1711503 + 873 WP_003028669.1 virulence factor BrkB family protein -
GZS04_RS08235 1711500..1711937 + 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
GZS04_RS08240 1711982..1712923 + 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
GZS04_RS08245 1713074..1713982 - 909 WP_046671350.1 alpha/beta hydrolase -
GZS04_RS08250 1714188..1714430 + 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
GZS04_RS08255 1714430..1714804 + 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
GZS04_RS08260 1714841..1715770 - 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
GZS04_RS08265 1715767..1716402 - 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
GZS04_RS08270 1716399..1717301 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T149307 WP_003028682.1 NZ_CP048382:1714430-1714804 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T149307 NZ_CP063996:c2196741-2196322 [Xanthomonas translucens pv. undulosa]
ATGATTGTCTTGGATACGAATGTCGTCTCCGAGGCGATGAAACCCGAACCCAACCCGGCCGTGCGAGCTTGGTTGAATGA
GCAGGTAGCGGAAACCCTCTACCTGTCCAGCGTGACGCTTGCCGAACTGCTGTTCGGCATCGGCGCGCTGCCGAACGGGA
AACGTAAAAAAGGCCTGGGCGAAGCCCTGGACGGATTGCTCGAACTGTTCAGCGAGCGGGTGCTGATGTTCGACACCGAA
GCAGCTCGCAATTATGCCGAGCTGGCGGTGAAGGCCCGTATAGCCGGGAAGGGTTTTCCAACACCCGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTGGCAACACGCGACACCAGCCCTTTTGAAGCGGCCGGGCTTACTGTTATCA
ATCCTTGGAACCATCGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT149307 WP_043018956.1 NZ_CP048382:1714188-1714430 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT149307 NZ_CP063996:c2196992-2196738 [Xanthomonas translucens pv. undulosa]
ATGGCAATCCTGACAGTGCGGAATGTGCCCGACGAGGTACATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTCCGCGAGATTCTGGAGAGTGCAGTTAAGTCACAAAAGCGCATCCGCATGGGCGACGCGTTGGCGG
AGCTTGGCCGCCGCGTAGGGCTAACAAATGATGATTTCGCAGTATTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCG
ATGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References