Detailed information of TA system TA149306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1291631..1292262
• Replicon: chromosome
• Accession: NZ_CP048382
• Organism: Citrobacter freundii strain 62

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GZS04_RS06260
• Protein ID: WP_046671263.1
• Coordinates: 1291987..1292262 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0J1MMS0
• Locus tag: GZS04_RS06255
• Protein ID: WP_003837808.1
• Coordinates: 1291631..1291990 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS04_RS06225	1286727..1287392	+	666	WP_003837818.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-
GZS04_RS06230	1287465..1288022	+	558	WP_046671264.1	DcrB family lipoprotein	-
GZS04_RS06235	1288026..1289243	-	1218	WP_032950436.1	MFS transporter	-
GZS04_RS06240	1289377..1290426	+	1050	WP_003023393.1	AI-2E family transporter	-
GZS04_RS06245	1290489..1291067	+	579	WP_003837810.1	4'-phosphopantetheinyl transferase AcpT	-
GZS04_RS06250	1291117..1291518	+	402	WP_003023387.1	nickel-responsive transcriptional regulator NikR	-
GZS04_RS06255	1291631..1291990	-	360	WP_003837808.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GZS04_RS06260	1291987..1292262	-	276	WP_046671263.1	type II toxin-antitoxin system HicA family toxin	Toxin
GZS04_RS06265	1292340..1293464	-	1125	WP_061547457.1	ABC transporter permease	-
GZS04_RS06270	1293464..1296211	-	2748	WP_162648218.1	ribosome-associated ATPase/putative transporter RbbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10238.90 Da
• Isoelectric Point: 10.7844

>T149306 WP_046671263.1 NZ_CP048382:c1292262-1291987 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIRWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T149306 NZ_CP063993:2883186-2883380 [Xanthomonas translucens pv. undulosa]
ATGCCCCCGAAAATCCGCGACGTGCTGCAACAACTCAAGGACGCAGGCTGGCAGCAAGTCGGCCAGACCGGCAGCCACCG
CCAGTTCAAGCATCCTGTCTACCCAGGCCGCGTGACAGTGGCCGGCAAGCCGAGCGATGATGTGGCTCCCGGCACCATGA
ACAGTATCCTGAAGCAAGCCGGCCTGAAGAGGTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13198.98 Da
• Isoelectric Point: 4.5462

>AT149306 WP_003837808.1 NZ_CP048382:c1291990-1291631 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT149306 NZ_CP063993:2883386-2883598 [Xanthomonas translucens pv. undulosa]
ATGCGCTACGCCGTCGTCATTGAGCAAGCGGGCAACAACTACTCCGCCTACGTGCCGGATCTGGCGGGGTGTGTGGCGAC
GGGCGCGACGACCGCGGAGGTGGCAGGATTGATCCGCGAGGCGATTGAGCTGCATCTGGCTGGCATGCGTGAGGATGGGG
AAGTGATTCCGCTGCCGTCGAGTCAGGTTGAGTATGTGGATGTGGCGGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MMS0