Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 64261..64786 Replicon plasmid p38_A-OXA140
Accession NZ_CP048377
Organism Escherichia coli strain 38

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag GZS06_RS23080 Protein ID WP_001159868.1
Coordinates 64261..64566 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A223LLB0
Locus tag GZS06_RS23085 Protein ID WP_023909027.1
Coordinates 64568..64786 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS06_RS23065 60171..61337 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
GZS06_RS23070 61925..62680 - 756 WP_000852146.1 replication initiation protein RepE -
GZS06_RS23075 63454..64260 - 807 WP_000016982.1 site-specific integrase -
GZS06_RS23080 64261..64566 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
GZS06_RS23085 64568..64786 - 219 WP_023909027.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GZS06_RS23090 65420..65617 + 198 WP_000215657.1 hypothetical protein -
GZS06_RS23095 65614..65898 - 285 WP_000642771.1 hypothetical protein -
GZS06_RS23100 65918..67051 - 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / sul1 / qacE / aadA5 / blaNDM-5 / aadA2 / dfrA12 / blaTEM-1B / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 - 1..86996 86996


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T149302 WP_001159868.1 NZ_CP048377:c64566-64261 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T149302 NZ_CP063993:1381569-1381820 [Xanthomonas translucens pv. undulosa]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAAGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.31 Da        Isoelectric Point: 4.7405

>AT149302 WP_023909027.1 NZ_CP048377:c64786-64568 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTIQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT149302 NZ_CP063993:1381817-1382074 [Xanthomonas translucens pv. undulosa]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACTACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACCGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACATCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLB0

References