Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3485573..3486191 Replicon chromosome
Accession NZ_CP048376
Organism Escherichia coli strain 38

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GZS06_RS16720 Protein ID WP_001291435.1
Coordinates 3485973..3486191 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GZS06_RS16715 Protein ID WP_000344800.1
Coordinates 3485573..3485947 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS06_RS16705 3480662..3481855 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GZS06_RS16710 3481878..3485027 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GZS06_RS16715 3485573..3485947 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GZS06_RS16720 3485973..3486191 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GZS06_RS16725 3486363..3486914 + 552 WP_000102568.1 maltose O-acetyltransferase -
GZS06_RS16730 3487030..3487500 + 471 WP_000136192.1 YlaC family protein -
GZS06_RS16735 3487664..3489214 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GZS06_RS16740 3489256..3489609 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GZS06_RS16750 3489988..3490299 + 312 WP_000409911.1 MGMT family protein -
GZS06_RS16755 3490330..3490902 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149296 WP_001291435.1 NZ_CP048376:3485973-3486191 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T149296 NZ_CP063992:c2144058-2143840 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT149296 WP_000344800.1 NZ_CP048376:3485573-3485947 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT149296 NZ_CP063992:c2144459-2144085 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References