Detailed information of TA system TA149295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2804746..2806466
• Replicon: chromosome
• Accession: NZ_CP048376
• Organism: Escherichia coli strain 38

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: GZS06_RS13495
• Protein ID: WP_000312833.1
• Coordinates: 2804746..2805402 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: GZS06_RS13500
• Protein ID: WP_000634203.1
• Coordinates: 2805399..2806466 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS06_RS13480	2800661..2800900	+	240	WP_024224214.1	hypothetical protein	-
GZS06_RS13485	2800940..2803378	+	2439	WP_032180025.1	type I restriction-modification system subunit M	-
GZS06_RS13490	2803390..2804733	+	1344	WP_032180130.1	restriction endonuclease subunit S	-
GZS06_RS13495	2804746..2805402	+	657	WP_000312833.1	DUF4433 domain-containing protein	Toxin
GZS06_RS13500	2805399..2806466	+	1068	WP_000634203.1	macro domain-containing protein	Antitoxin
GZS06_RS13505	2806485..2809580	+	3096	WP_000108736.1	HsdR family type I site-specific deoxyribonuclease	-
GZS06_RS13510	2809580..2810296	+	717	WP_001122107.1	M48 family metallopeptidase	-
GZS06_RS13515	2810641..2811255	+	615	Protein_2648	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	blaCMY-2	-	2787874..2832569	44695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T149295 WP_000312833.1 NZ_CP048376:2804746-2805402 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T149295 NZ_CP063992:1516270-1516803 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT149295 WP_000634203.1 NZ_CP048376:2805399-2806466 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT149295 NZ_CP063992:1515993-1516259 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9