Detailed information of TA system TA149267

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3679464..3680158
• Replicon: chromosome
• Accession: NZ_CP048371
• Organism: Escherichia coli strain 163

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: GZS08_RS17565
• Protein ID: WP_001094398.1
• Coordinates: 3679464..3679832 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: GZS08_RS17570
• Protein ID: WP_077249034.1
• Coordinates: 3679853..3680158 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS08_RS17545	3674637..3675593	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
GZS08_RS17550	3675590..3677788	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
GZS08_RS17555	3677798..3678754	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
GZS08_RS17560	3678805..3679143	+	339	Protein_3435	LysR family transcriptional regulator	-
GZS08_RS17565	3679464..3679832	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
GZS08_RS17570	3679853..3680158	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS08_RS17575	3680262..3680906	-	645	WP_000086755.1	hypothetical protein	-
GZS08_RS17580	3680925..3681146	-	222	WP_000692345.1	DUF987 domain-containing protein	-
GZS08_RS17585	3681209..3681685	-	477	WP_001186774.1	RadC family protein	-
GZS08_RS17590	3681701..3682174	-	474	WP_000855059.1	antirestriction protein	-
GZS08_RS17595	3682516..3683337	-	822	WP_001234565.1	DUF945 domain-containing protein	-
GZS08_RS17600	3683438..3683646	-	209	Protein_3443	DUF905 family protein	-
GZS08_RS17605	3683747..3684202	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T149267 WP_001094398.1 NZ_CP048371:c3679832-3679464 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T149267 NZ_CP063983:c830720-830343 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT149267 WP_077249034.1 NZ_CP048371:c3680158-3679853 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT149267 NZ_CP063983:c831177-830797 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCGCCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4