149242

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 55059..55584 Replicon plasmid pC-F-164_A-OXA140
Accession NZ_CP048368
Organism Escherichia coli strain 164

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag GZS07_RS23775 Protein ID WP_001159871.1
Coordinates 55279..55584 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag GZS07_RS23770 Protein ID WP_000813630.1
Coordinates 55059..55277 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS07_RS23745 50270..50955 + 686 Protein_56 IS1 family transposase -
GZS07_RS23750 51209..52231 - 1023 WP_000361402.1 helicase UvrD -
GZS07_RS23755 52216..53781 - 1566 WP_001128474.1 AAA family ATPase -
GZS07_RS23760 53856..54272 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
GZS07_RS23765 54269..54499 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GZS07_RS23770 55059..55277 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GZS07_RS23775 55279..55584 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
GZS07_RS23780 55585..56391 + 807 WP_000016970.1 site-specific integrase -
GZS07_RS23785 57113..57868 + 756 WP_000852146.1 replication initiation protein RepE -
GZS07_RS23790 58456..59622 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) - 1..132482 132482


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T149242 WP_001159871.1 NZ_CP048368:55279-55584 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T149242 NZ_CP063954:4946508-4946891 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT149242 WP_000813630.1 NZ_CP048368:55059-55277 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT149242 NZ_CP063954:4946266-4946508 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References