Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4170609..4171185 Replicon chromosome
Accession NZ_CP048367
Organism Escherichia coli strain 164

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag GZS07_RS20030 Protein ID WP_001297643.1
Coordinates 4170898..4171185 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag GZS07_RS20025 Protein ID WP_000063160.1
Coordinates 4170609..4170911 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS07_RS20010 4167205..4169355 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
GZS07_RS20015 4169405..4169608 + 204 WP_000467859.1 YbdD/YjiX family protein -
GZS07_RS20020 4169619..4170575 + 957 WP_001297640.1 GTPase -
GZS07_RS20025 4170609..4170911 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
GZS07_RS20030 4170898..4171185 - 288 WP_001297643.1 BrnT family toxin Toxin
GZS07_RS20035 4171384..4172081 - 698 Protein_3914 IS1 family transposase -
GZS07_RS20040 4172396..4173628 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
GZS07_RS20045 4173669..4174949 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4171384..4171593 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T149238 WP_001297643.1 NZ_CP048367:c4171185-4170898 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T149238 NZ_CP063954:c4095013-4094603 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATTAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT149238 WP_000063160.1 NZ_CP048367:c4170911-4170609 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT149238 NZ_CP063954:c4095260-4094994 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References