Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3537984..3538602 Replicon chromosome
Accession NZ_CP048367
Organism Escherichia coli strain 164

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GZS07_RS16965 Protein ID WP_001291435.1
Coordinates 3538384..3538602 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GZS07_RS16960 Protein ID WP_000344800.1
Coordinates 3537984..3538358 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS07_RS16950 3533073..3534266 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GZS07_RS16955 3534289..3537438 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GZS07_RS16960 3537984..3538358 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GZS07_RS16965 3538384..3538602 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GZS07_RS16970 3538774..3539325 + 552 WP_000102564.1 maltose O-acetyltransferase -
GZS07_RS16975 3539441..3539911 + 471 WP_000136192.1 YlaC family protein -
GZS07_RS16980 3540075..3541625 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GZS07_RS16985 3541667..3542020 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GZS07_RS16995 3542399..3542710 + 312 WP_000409911.1 MGMT family protein -
GZS07_RS17000 3542741..3543313 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149234 WP_001291435.1 NZ_CP048367:3538384-3538602 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T149234 NZ_CP063954:787158-787475 [Klebsiella variicola]
ATGTTCAGAATAGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT149234 WP_000344800.1 NZ_CP048367:3537984-3538358 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT149234 NZ_CP063954:787468-787755 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATGTCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References