Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2505979..2506617 Replicon chromosome
Accession NZ_CP048367
Organism Escherichia coli strain 164

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag GZS07_RS12000 Protein ID WP_001447010.1
Coordinates 2506441..2506617 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GZS07_RS11995 Protein ID WP_001270286.1
Coordinates 2505979..2506395 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS07_RS11975 2501131..2502072 - 942 WP_001251304.1 ABC transporter permease -
GZS07_RS11980 2502073..2503086 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GZS07_RS11985 2503104..2504249 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
GZS07_RS11990 2504494..2505900 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
GZS07_RS11995 2505979..2506395 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GZS07_RS12000 2506441..2506617 - 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GZS07_RS12005 2506839..2507069 + 231 WP_000494244.1 YncJ family protein -
GZS07_RS12010 2507161..2509122 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GZS07_RS12015 2509195..2509731 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GZS07_RS12020 2509784..2510998 + 1215 WP_162664787.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T149233 WP_001447010.1 NZ_CP048367:c2506617-2506441 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T149233 NZ_CP063954:c647344-647126 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT149233 WP_001270286.1 NZ_CP048367:c2506395-2505979 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT149233 NZ_CP063954:c647745-647371 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References