Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 133828..134471 Replicon plasmid p53_A
Accession NZ_CP048360
Organism Escherichia coli strain 53

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag GZS09_RS24250 Protein ID WP_001034044.1
Coordinates 134055..134471 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag GZS09_RS24245 Protein ID WP_001261286.1
Coordinates 133828..134058 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS09_RS24220 129437..130141 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
GZS09_RS24225 130459..131214 - 756 WP_000852146.1 replication initiation protein RepE -
GZS09_RS24230 131936..132742 - 807 WP_000016970.1 site-specific integrase -
GZS09_RS24235 132743..133048 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
GZS09_RS24240 133050..133268 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
GZS09_RS24245 133828..134058 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GZS09_RS24250 134055..134471 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
GZS09_RS24255 134546..136111 + 1566 WP_001128474.1 AAA family ATPase -
GZS09_RS24260 136096..137118 + 1023 WP_000361402.1 helicase UvrD -
GZS09_RS24265 137372..138058 - 687 Protein_177 IS1 family transposase -
GZS09_RS24270 138169..139026 - 858 WP_000968139.1 iron/manganese ABC transporter permease subunit SitD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / blaDHA-1 / qnrB4 / sitABCD - 1..144585 144585


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T149208 WP_001034044.1 NZ_CP048360:134055-134471 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T149208 NZ_CP063945:3019141-3019359 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT149208 WP_001261286.1 NZ_CP048360:133828-134058 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT149208 NZ_CP063945:3018740-3019114 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References