Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4163399..4163975 Replicon chromosome
Accession NZ_CP048359
Organism Escherichia coli strain 53

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag GZS09_RS19945 Protein ID WP_001297643.1
Coordinates 4163688..4163975 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag GZS09_RS19940 Protein ID WP_000063156.1
Coordinates 4163399..4163701 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS09_RS19925 4159995..4162145 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
GZS09_RS19930 4162195..4162398 + 204 WP_000467859.1 YbdD/YjiX family protein -
GZS09_RS19935 4162409..4163365 + 957 WP_001393094.1 GTPase -
GZS09_RS19940 4163399..4163701 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
GZS09_RS19945 4163688..4163975 - 288 WP_001297643.1 BrnT family toxin Toxin
GZS09_RS19950 4164174..4164871 - 698 Protein_3908 IS1 family transposase -
GZS09_RS19955 4165197..4166429 + 1233 WP_001137021.1 multidrug efflux MFS transporter MdtM -
GZS09_RS19960 4166470..4167750 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4164174..4164383 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T149195 WP_001297643.1 NZ_CP048359:c4163975-4163688 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T149195 NZ_CP063943:1443617-1443719 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT149195 WP_000063156.1 NZ_CP048359:c4163701-4163399 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT149195 NZ_CP063943:c1443726-1443581 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References