149190

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3602404..3603241 Replicon chromosome
Accession NZ_CP048359
Organism Escherichia coli strain 53

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag GZS09_RS17345 Protein ID WP_000227784.1
Coordinates 3602699..3603241 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag GZS09_RS17340 Protein ID WP_001297137.1
Coordinates 3602404..3602715 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS09_RS17315 3597424..3598371 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
GZS09_RS17320 3598393..3600384 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
GZS09_RS17325 3600374..3600988 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
GZS09_RS17330 3600988..3601317 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
GZS09_RS17335 3601329..3602219 + 891 WP_000971336.1 heme o synthase -
GZS09_RS17340 3602404..3602715 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
GZS09_RS17345 3602699..3603241 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
GZS09_RS17350 3603297..3604232 - 936 WP_001297127.1 sel1 repeat family protein -
GZS09_RS17355 3604640..3606004 + 1365 WP_001000978.1 MFS transporter -
GZS09_RS17360 3606132..3606623 - 492 WP_001138904.1 nucleotide binding protein YajQ -
GZS09_RS17365 3606791..3607702 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T149190 WP_000227784.1 NZ_CP048359:3602699-3603241 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T149190 NZ_CP063942:c3774193-3774091 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT149190 WP_001297137.1 NZ_CP048359:3602404-3602715 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT149190 NZ_CP063942:3774087-3774234 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References