Detailed information of TA system TA149177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1326504..1327129
• Replicon: chromosome
• Accession: NZ_CP048359
• Organism: Escherichia coli strain 53

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GZS09_RS06410
• Protein ID: WP_000911330.1
• Coordinates: 1326731..1327129 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GZS09_RS06405
• Protein ID: WP_000450524.1
• Coordinates: 1326504..1326731 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS09_RS06380	1322307..1322777	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
GZS09_RS06385	1322777..1323349	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GZS09_RS06390	1323495..1324373	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GZS09_RS06395	1324390..1325424	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
GZS09_RS06400	1325637..1326350	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GZS09_RS06405	1326504..1326731	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GZS09_RS06410	1326731..1327129	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GZS09_RS06415	1327276..1328139	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
GZS09_RS06420	1328154..1330169	+	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
GZS09_RS06425	1330243..1330941	+	699	WP_000679823.1	esterase	-
GZS09_RS06430	1331051..1331251	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T149177 WP_000911330.1 NZ_CP048359:1326731-1327129 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T149177 NZ_CP063939:c2402130-2401813 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT149177 WP_000450524.1 NZ_CP048359:1326504-1326731 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT149177 NZ_CP063939:c2401820-2401533 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33