Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3493980..3494598 Replicon chromosome
Accession NZ_CP048344
Organism Escherichia coli strain 124

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GZS11_RS16805 Protein ID WP_001291435.1
Coordinates 3494380..3494598 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GZS11_RS16800 Protein ID WP_000344800.1
Coordinates 3493980..3494354 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS11_RS16790 3489348..3490262 + 915 Protein_3281 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GZS11_RS16795 3490285..3493434 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GZS11_RS16800 3493980..3494354 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GZS11_RS16805 3494380..3494598 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GZS11_RS16810 3494770..3495321 + 552 WP_000102568.1 maltose O-acetyltransferase -
GZS11_RS16815 3495437..3495907 + 471 WP_000136192.1 YlaC family protein -
GZS11_RS16820 3496071..3497621 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GZS11_RS16825 3497663..3498016 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GZS11_RS16835 3498395..3498706 + 312 WP_000409911.1 MGMT family protein -
GZS11_RS16840 3498737..3499309 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149162 WP_001291435.1 NZ_CP048344:3494380-3494598 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T149162 NZ_CP063935:c57180-56764 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCATTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT149162 WP_000344800.1 NZ_CP048344:3493980-3494354 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT149162 NZ_CP063935:c57407-57177 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGTGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References