Detailed information of TA system TA149149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1321590..1322215
• Replicon: chromosome
• Accession: NZ_CP048344
• Organism: Escherichia coli strain 124

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GZS11_RS06360
• Protein ID: WP_000911330.1
• Coordinates: 1321817..1322215 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GZS11_RS06355
• Protein ID: WP_000450524.1
• Coordinates: 1321590..1321817 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS11_RS06330	1317393..1317863	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
GZS11_RS06335	1317863..1318435	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GZS11_RS06340	1318581..1319459	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GZS11_RS06345	1319476..1320510	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
GZS11_RS06350	1320723..1321436	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GZS11_RS06355	1321590..1321817	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GZS11_RS06360	1321817..1322215	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GZS11_RS06365	1322362..1323225	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
GZS11_RS06370	1323240..1325255	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
GZS11_RS06375	1325329..1325670	+	342	Protein_1245	esterase	-
GZS11_RS06380	1325752..1326498	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T149149 WP_000911330.1 NZ_CP048344:1321817-1322215 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T149149 NZ_CP063934:c239811-239278 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT149149 WP_000450524.1 NZ_CP048344:1321590-1321817 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT149149 NZ_CP063934:c240088-239822 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33