Detailed information of TA system TA149141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2032..2585
• Replicon: plasmid p142_B
• Accession: NZ_CP048339
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GZS12_RS25220
• Protein ID: WP_162646453.1
• Coordinates: 2271..2585 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: GZS12_RS25215
• Protein ID: WP_162646452.1
• Coordinates: 2032..2268 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS12_RS25215	2032..2268	+	237	WP_162646452.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GZS12_RS25220	2271..2585	+	315	WP_162646453.1	CcdB family protein	Toxin
GZS12_RS25225	2870..3379	-	510	WP_162646454.1	MucR family transcriptional regulator	-
GZS12_RS25230	3427..5514	-	2088	WP_162646455.1	conjugal transfer protein TrbC	-
GZS12_RS25235	5527..6477	-	951	WP_004206886.1	DsbC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..59239	59239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11410.29 Da
• Isoelectric Point: 7.1309

>T149141 WP_162646453.1 NZ_CP048339:2271-2585 [Escherichia coli]
MQFTVYSNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPAEKYPGSTRPERLVPLVRLTDGKEYVVMTHEMASIPACSLG
GAFCDASFHRTQVKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T149141 NZ_CP063933:c1424760-1424428 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGTGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8984.96 Da
• Isoelectric Point: 4.8040

>AT149141 WP_162646452.1 NZ_CP048339:2032-2268 [Escherichia coli]
MATTQRATQSVTMTVERALLSRAREAGINLSATLTAALDAELRQYEVKKWQEENYEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT149141 NZ_CP063933:c1425018-1424761 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA