Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 133139..133782 Replicon plasmid p142_A-OXA181
Accession NZ_CP048338
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag GZS12_RS25110 Protein ID WP_001034044.1
Coordinates 133366..133782 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag GZS12_RS25105 Protein ID WP_001261286.1
Coordinates 133139..133369 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS12_RS25090 128276..128506 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GZS12_RS25095 128503..128919 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
GZS12_RS25100 128964..132758 - 3795 WP_001144732.1 hypothetical protein -
GZS12_RS25105 133139..133369 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GZS12_RS25110 133366..133782 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
GZS12_RS25115 133857..135415 + 1559 Protein_162 AAA family ATPase -
GZS12_RS25120 135400..136422 + 1023 WP_000361402.1 helicase UvrD -
GZS12_RS25125 136676..137373 - 698 Protein_164 IS1 family transposase -
GZS12_RS25130 137729..138643 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-181 / qnrS1 / qnrB4 / blaDHA-1 / sul1 / mph(A) / catA1 / tet(B) / sitABCD iucA / iucB / iucB / iucC / iucD / iutA 1..155486 155486
- flank IS/Tn sitABCD - 136676..141178 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T149140 WP_001034044.1 NZ_CP048338:133366-133782 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T149140 NZ_CP063933:c1307380-1307278 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT149140 WP_001261286.1 NZ_CP048338:133139-133369 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT149140 NZ_CP063933:1307272-1307416 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References