Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 123787..124312 Replicon plasmid p142_A-OXA181
Accession NZ_CP048338
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag GZS12_RS25060 Protein ID WP_001159868.1
Coordinates 123787..124092 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag GZS12_RS25065 Protein ID WP_000813634.1
Coordinates 124094..124312 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS12_RS25045 119697..120863 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
GZS12_RS25050 121451..122206 - 756 WP_024946706.1 replication initiation protein RepE -
GZS12_RS25055 122980..123786 - 807 WP_000016982.1 site-specific integrase -
GZS12_RS25060 123787..124092 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
GZS12_RS25065 124094..124312 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GZS12_RS25070 124879..125391 + 513 WP_000151784.1 hypothetical protein -
GZS12_RS25075 125425..126558 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
GZS12_RS25080 126725..127498 - 774 WP_000905949.1 hypothetical protein -
GZS12_RS25085 127511..128011 - 501 WP_000528931.1 hypothetical protein -
GZS12_RS25090 128276..128506 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GZS12_RS25095 128503..128919 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-181 / qnrS1 / qnrB4 / blaDHA-1 / sul1 / mph(A) / catA1 / tet(B) / sitABCD iucA / iucB / iucB / iucC / iucD / iutA 1..155486 155486


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T149138 WP_001159868.1 NZ_CP048338:c124092-123787 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T149138 NZ_CP063932:5491916-5492299 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT149138 WP_000813634.1 NZ_CP048338:c124312-124094 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT149138 NZ_CP063932:5491674-5491916 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References