Detailed information of TA system TA149131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5213..5772
• Replicon: plasmid p142_A-OXA181
• Accession: NZ_CP048338
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: GZS12_RS24340
• Protein ID: WP_000421272.1
• Coordinates: 5213..5488 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: GZS12_RS24345
• Protein ID: WP_001387467.1
• Coordinates: 5488..5772 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS12_RS24310	1263..2003	-	741	WP_001066953.1	tyrosine-type recombinase/integrase	-
GZS12_RS24315	2124..2306	-	183	WP_042344623.1	hypothetical protein	-
GZS12_RS24320	2649..2956	-	308	Protein_3	type II toxin-antitoxin system ParD family antitoxin	-
GZS12_RS24325	3449..4618	+	1170	WP_001072355.1	hypothetical protein	-
GZS12_RS24330	4651..4779	+	129	Protein_5	IS481 family transposase	-
GZS12_RS24335	4814..5107	-	294	WP_001105060.1	hypothetical protein	-
GZS12_RS24340	5213..5488	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZS12_RS24345	5488..5772	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
GZS12_RS24350	6377..7129	+	753	WP_000562172.1	IclR family transcriptional regulator	-
GZS12_RS24355	7175..8140	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
GZS12_RS24360	8173..8553	-	381	WP_024946717.1	Rid family detoxifying hydrolase	-
GZS12_RS24365	8578..9468	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
GZS12_RS24370	9701..9895	-	195	WP_000796505.1	hypothetical protein	-
GZS12_RS24375	9869..10309	+	441	Protein_14	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-181 / qnrS1 / qnrB4 / blaDHA-1 / sul1 / mph(A) / catA1 / tet(B) / sitABCD	iucA / iucB / iucB / iucC / iucD / iutA	1..155486	155486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T149131 WP_000421272.1 NZ_CP048338:c5488-5213 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T149131 NZ_CP063932:1302615-1302932 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT149131 WP_001387467.1 NZ_CP048338:c5772-5488 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT149131 NZ_CP063932:1302925-1303212 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880